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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant part in promoting cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been described. Two further splice variants were described but comprehensive nucleotide sequence information and facts will not be but available.

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Blocking peptides are peptides that bind specifically towards the target antibody and block antibody binding. These peptide ordinarily incorporates the epitope recognized because of the antibody.

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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the very best effects are...

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This guide outlines more doable optimization of cellular and immunoassay parameters to ensure the best possible results are...

Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining with the blocked antibody more info as opposed to the antibody by yourself, one can see which staining is certain.

Goal Info Cyclin E belongs towards the extremely conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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